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ProductsBack/Smad1 (913C1b): m-IgG1 BP-HRP Bundle

Smad1 (913C1b): m-IgG1 BP-HRP Bundle

SC-542626
Santa Cruz Biotechnology
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD1
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    Smad1 (913C1b): m-IgG1 BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4086
  • Target name
    SMAD1
  • Target description
    SMAD family member 1
  • Target synonyms
    BSP-1, BSP1, JV4-1, JV41, MADH1, MADR1, mothers against decapentaplegic homolog 1, MAD, mothers against decapentaplegic homolog 1, Mad-related protein 1, SMAD, mothers against DPP homolog 1, TGF-beta signaling protein 1, mothers against DPP homolog 1, transforming growth factor-beta signaling protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Hela cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Smad1 + Smad5 Antibody(bs-1619R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Product group Antibodies
References
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Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.
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