![WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83612 SMAD2 antibody [2C10]. Loading : 5 ug per lane WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83612 SMAD2 antibody [2C10]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX83612/GTX83612_3767_WB_w_23061420_337.webp)
WB analysis of HEK293T cells transfected with SMAD2 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83612 SMAD2 antibody [2C10]. Loading : 5 ug per lane
SMAD2 antibody [2C10]
GTX83612
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSMAD2
Overview
- SupplierGeneTex
- Product NameSMAD2 antibody [2C10]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID2C10
- Concentration0.95 mg/ml
- ConjugateUnconjugated
- Gene ID4087
- Target nameSMAD2
- Target descriptionSMAD family member 2
- Target synonymsCHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, mothers against decapentaplegic homolog 2, MAD homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
- HostMouse
- IsotypeIgG2b
- Protein IDQ15796
- Protein NameMothers against decapentaplegic homolog 2
- Scientific DescriptionTranscriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![ICC/IF analysis of COS7 cells transiently transfected with SMAD2 plasmid using GTX83612 SMAD2 antibody [2C10]. ICC/IF analysis of COS7 cells transiently transfected with SMAD2 plasmid using GTX83612 SMAD2 antibody [2C10].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83612/GTX83612_738_ICCIF_w_23061420_559.webp)
![FACS analysis of Jurkat cells using GTX83612 SMAD2 antibody [2C10]. Red : Primary antibody Blue : Negative control antibody FACS analysis of Jurkat cells using GTX83612 SMAD2 antibody [2C10]. Red : Primary antibody Blue : Negative control antibody](https://www.genetex.com/upload/website/prouct_img/normal/GTX83612/GTX83612_90_FACS_w_23061420_618.webp)



![FACS analysis of HeLa cells using GTX01034 SMAD2 (phospho Ser250) antibody [SD207-1]. Red : primary antibody Black : unlabelled control Dilution : 1:50](https://www.genetex.com/upload/website/prouct_img/normal/GTX01034/GTX01034_20200303_FACS_237_w_23053121_650.webp)
![WB analysis of various cell lines using GTX83613 SMAD2 antibody [4G7]. Loading : 35 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX83613/GTX83613_3688_WB_w_23061420_449.webp)