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ProductsBack/SMAD2 Mouse Monoclonal Antibody [Clone ID: OTI4G7]
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SMAD2 plasmid (RC204604, hSMAD2), mouse SMAD2 plasmid (MR207463, mSMAD2) using anti-SMAD2 antibody TA501777 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SMAD2 plasmid (RC204604, hSMAD2), mouse SMAD2 plasmid (MR207463, mSMAD2) using anti-SMAD2 antibody TA501777 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).
Figure A, Western blot analysis of overexpressed lysates (15ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SMAD2 plasmid (RC204604, hSMAD2), mouse SMAD2 plasmid (MR207463, mSMAD2) using anti-SMAD2 antibody TA501777 (1:10000@1mg/ml). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:10000@1mg/ml).

SMAD2 Mouse Monoclonal Antibody [Clone ID: OTI4G7]

CF501777
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SMAD2 Mouse Monoclonal Antibody [Clone ID: OTI4G7]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4G7
  • Gene ID4087
  • Target name
    SMAD2
  • Target description
    SMAD family member 2
  • Target synonyms
    CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2, mothers against decapentaplegic homolog 2, MAD homolog 2, SMAD, mothers against DPP homolog 2, Sma- and Mad-related protein 2, mother against DPP homolog 2
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ15796
  • Protein Name
    Mothers against decapentaplegic homolog 2
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SMAD2 mouse monoclonal antibody, clone OTI4G7 (formerly 4G7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: rat liver tissue lysates Lane 3: mouse testicular tissue lysates Lane 4: mouse heart tissue lysates Lane 5: mouse lung tissue lysates Lane 6: mouse lung tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.
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