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ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.
ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.
ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.

SMAD2 (phospho Thr8) antibody

GTX25487
GeneTex
ApplicationsFlow Cytometry, Western Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
TargetSMAD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMAD2 (phospho Thr8) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    FACS: 1:20. ChIP assay: 10microl. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ChIP Chromatin ImmunoPrecipitation
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4087
  • Target name
    SMAD2
  • Target description
    SMAD family member 2
  • Target synonyms
    hMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; MADH2; MADR2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15796
  • Protein Name
    Mothers against decapentaplegic homolog 2
  • Scientific Description
    The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMAD2 using anti-SMAD2 antibody (A00090-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates Lane 2: rat liver tissue lysates Lane 3: mouse testicular tissue lysates Lane 4: mouse heart tissue lysates Lane 5: mouse lung tissue lysates Lane 6: mouse lung tissue lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD2 antigen affinity purified polyclonal antibody (Catalog # A00090-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMAD2 at approximately 60KD. The expected band size for SMAD2 is at 52KD.
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