ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.
ChIP analysis of 2 million Jurkat cells treated with 50ng/ml of TGF-beta for one hour using GTX25487 SMAD2 (phospho Thr8) antibody. Normal Rabbit IgG was used as a negative IP control. The precipitated DNA was detected by PCR with primer set targeting to the promoter of ID1 gene as positive control and the inactive SAT2 used as negative control target.
SMAD2 (phospho Thr8) antibody - Orthogonal Validated
GTX25487
ApplicationsFlow Cytometry, Western Blot, ChIP Chromatin ImmunoPrecipitation
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSMAD2
Overview
- SupplierGeneTex
- Product NameSMAD2 (phospho Thr8) antibody - Orthogonal Validated
- Delivery Days Customer9
- ApplicationsFlow Cytometry, Western Blot, ChIP Chromatin ImmunoPrecipitation
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID4087
- Target nameSMAD2
- Target descriptionSMAD family member 2
- Target synonymshMAD-2; hSMAD2; JV18; JV18-1; MAD homolog 2; MADH2; MADR2; mother against DPP homolog 2; mothers against decapentaplegic homolog 2; Sma- and Mad-related protein 2; SMAD, mothers against DPP homolog 2
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene mothers against decapentaplegic (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, May 2012]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203