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ProductsBack/SMARCC1 antibody [2A4-H7-C12]
WB analysis of K562, Jurkat and HeLa lysates using SMARCC1 antibody at a dilution of 1:1,000.
WB analysis of K562, Jurkat and HeLa lysates using SMARCC1 antibody at a dilution of 1:1,000.
WB analysis of K562, Jurkat and HeLa lysates using SMARCC1 antibody at a dilution of 1:1,000.

SMARCC1 antibody [2A4-H7-C12]

GTX49155
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetSMARCC1
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMARCC1 antibody [2A4-H7-C12]
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A4-H7-C12
  • Conjugate
    Unconjugated
  • Gene ID6599
  • Target name
    SMARCC1
  • Target description
    SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1
  • Target synonyms
    BAF155; BRG1-associated factor 155; chromatin remodeling complex BAF155 subunit; CRACC1; mammalian chromatin remodeling complex BRG1-associated factor 155; Rsc8; SRG3; SWI/SNF complex 155 kDa subunit; SWI/SNF complex subunit SMARCC1; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1; SWI3
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ92922
  • Protein Name
    SWI/SNF complex subunit SMARCC1
  • Scientific Description
    The protein encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and contains a predicted leucine zipper motif typical of many transcription factors. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMARCC1/BAF155 using anti-SMARCC1/BAF155 antibody (A03303-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hel whole cell lysates, Lane 5: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCC1/BAF155 antigen affinity purified polyclonal antibody (Catalog # A03303-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCC1/BAF155 at approximately 160 kDa. The expected band size for SMARCC1/BAF155 is at 160 kDa.
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