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ProductsBack/SMARCC1 / SWI3 Antibody

SMARCC1 / SWI3 Antibody

LS-C814121
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSMARCC1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SMARCC1 / SWI3 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6599
  • Target name
    SMARCC1
  • Target description
    SWI/SNF related BAF chromatin remodeling complex subunit C1
  • Target synonyms
    BAF155, CRACC1, HYC5, Rsc8, SRG3, SWI3, SWI/SNF complex subunit SMARCC1, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF related, matrix associated, actin dependent regulator of chromatin subfamily c member 1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, chromatin remodeling complex BAF155 subunit, mammalian chromatin remodeling complex BRG1-associated factor 155
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMARCC1/BAF155 using anti-SMARCC1/BAF155 antibody (A03303-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human Hel whole cell lysates, Lane 5: human Raji whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMARCC1/BAF155 antigen affinity purified polyclonal antibody (Catalog # A03303-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMARCC1/BAF155 at approximately 160 kDa. The expected band size for SMARCC1/BAF155 is at 160 kDa.
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