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Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: SMC5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 129 kDa Observed band size: 145 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: SMC5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 129 kDa Observed band size: 145 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, 293T whole cell lysate All lanes: SMC5 antibody at 4.6microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 129 kDa Observed band size: 145 kDa

SMC5 Antibody

CSB-PA810294LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSMC5
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Overview

  • Supplier
    Cusabio
  • Product Name
    SMC5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23137
  • Target name
    SMC5
  • Target description
    structural maintenance of chromosomes 5
  • Target synonyms
    hSMC5; SMC protein 5; SMC-5; SMC5 structural maintenance of chromosomes 5-like 1; SMC5L1; structural maintenance of chromosomes protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IY18
  • Protein Name
    Structural maintenance of chromosomes protein 5
  • Scientific Description
    Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Required for recruitment of telomeres to PML nuclear bodies. Required for sister chromatid cohesion during prometaphase and mitotic progression; the function seems to be independent of SMC6. SMC5-SMC6 complex may prevent transcription of episomal DNA, such as circular viral DNA genome (PubMed:26983541).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SMC5 using anti-SMC5 antibody (A01285). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC5 antigen affinity purified polyclonal antibody (Catalog # A01285) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC5 at approximately 130 kDa. The expected band size for SMC5 is at 129 kDa.
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