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ProductsBack/SMC5 antibody [N2N3]
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with SMC5 antibody [N2N3] (GTX115669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SMC5 antibody [N2N3]

GTX115669
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSMC5
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Overview

  • Supplier
    GeneTex
  • Product Name
    SMC5 antibody [N2N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23137
  • Target name
    SMC5
  • Target description
    structural maintenance of chromosomes 5
  • Target synonyms
    ATELS2, SMC5L1, structural maintenance of chromosomes protein 5, SMC protein 5, SMC-5, SMC5 structural maintenance of chromosomes 5-like 1, hSMC5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8IY18
  • Protein Name
    Structural maintenance of chromosomes protein 5
  • Scientific Description
    Core component of the SMC5-SMC6 complex, a complex involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SMC5 using anti-SMC5 antibody (A01285). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: rat testis tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC5 antigen affinity purified polyclonal antibody (Catalog # A01285) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SMC5 at approximately 130 kDa. The expected band size for SMC5 is at 129 kDa.
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