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SOCS-3 Antibody (YA3498, Mouse)

HY-P83801
MedChem Express
TargetSOCS3
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SOCS-3 Antibody (YA3498, Mouse)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID9021
  • Target name
    SOCS3
  • Target description
    suppressor of cytokine signaling 3
  • Target synonyms
    ATOD4; CIS3; Cish3; cytokine-inducible SH2 protein 3; SOCS-3; SSI3; SSI-3; STAT-induced STAT inhibitor 3; suppressor of cytokine signaling 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    SOCS-3 Antibody (YA3498) is a mouse-derived and non-conjugated IgG1 antibody, targeting to SOCS3. It can be applicated for WB, IHC-P, ICC/IF, FC, ELISA assays, in the background of human.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SOCS3 using anti-SOCS3 antibody (A00274-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat skeletal muscle tissue lysates, Lane 4: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOCS3 antigen affinity purified polyclonal antibody (Catalog # A00274-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOCS3 at approximately 28-30KD. The expected band size for SOCS3 is at 28-30KD.
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