![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636934/GTX636934_44788_20220902_WB_B_22092119_512.webp)
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SOD2 (Acetyl Lys68) antibody [HL1460]
GTX636934
ApplicationsDot Blot, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetSOD2
Overview
- SupplierGeneTex
- Product NameSOD2 (Acetyl Lys68) antibody [HL1460]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsDot Blot, Western Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1460
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID6648
- Target nameSOD2
- Target descriptionsuperoxide dismutase 2
- Target synonymsGC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
- HostRabbit
- IsotypeIgG
- Protein IDP04179
- Protein NameSuperoxide dismutase [Mn], mitochondrial
- Scientific DescriptionThis gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636934/GTX636934_44788_20221007_WB_M_heart_22101319_444.webp)
![Dotblot analysis of anti-SOD2 (Acetyl Lys68) antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) varied dilution. Dotblot analysis of anti-SOD2 (Acetyl Lys68) antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) varied dilution.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636934/GTX636934_T-44662_20240422_Dot_24043002_612.webp)


![WB analysis of various samples using GTX09013 SOD2 antibody [GT1165]. Dilution : 1:1000 Loading : 25 μg](https://www.genetex.com/upload/website/prouct_img/normal/GTX09013/GTX09013_20200508_WB_w_23053123_761.webp)

![SOD2 antibody [GT582] detects SOD2 protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 μg A431 whole cell lysate/extract C. 30 μg HeLa whole cell lysate/extract | D. 30 μg HepG2 whole cell lysate/extract 12 % SDS-PAGE SOD2 antibody [GT582] (GTX630558) dilution: 1:5000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630558/GTX630558_41589_WB_w_23061202_990.webp)
![SOD2 antibody [GT1433] detects SOD2 protein by western blot analysis. A. 50 μg rat brain lysate/extract 12 % SDS-PAGE SOD2 antibody [GT1433] (GTX630559) dilution: 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX630559/GTX630559_41589_WB_R_brain_w_23061202_222.webp)
![SOD2 antibody [GT17112] detects SOD2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOD2 stained by SOD2 antibody [GT17112] (GTX630560) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX630560/GTX630560_41589_20180629_IHC-P_w_23061202_309.webp)
![SOD2 antibody [HL1483] detects SOD2 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. SOD2 stained by SOD2 antibody [HL1483] (GTX636957) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX636957/GTX636957_T-44669_20220617_IHC-P_22062121_897.webp)