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ProductsBack/SOD2 Mouse Monoclonal Antibody [Clone ID: OTI1H6]
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SOD2 plasmid (RC202330, hSOD2 v1), human SOD2 plasmid (RC212924, hSOD2 v2), mouse SOD2 plasmid (MR202568, mSOD2) using anti-SOD2 antibody TA501887 (1:5000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000)
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SOD2 plasmid (RC202330, hSOD2 v1), human SOD2 plasmid (RC212924, hSOD2 v2), mouse SOD2 plasmid (MR202568, mSOD2) using anti-SOD2 antibody TA501887 (1:5000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000)
Figure A, Western blot analysis of overexpressed lysates (25ug per lane) from HEK293T cells transfected with empty plasmid (PS100001, NC), human SOD2 plasmid (RC202330, hSOD2 v1), human SOD2 plasmid (RC212924, hSOD2 v2), mouse SOD2 plasmid (MR202568, mSOD2) using anti-SOD2 antibody TA501887 (1:5000). Figure B, Western blot analysis of the same samples as figure A with anti-DDK antibody (TA180144, 1:5000)

SOD2 Mouse Monoclonal Antibody [Clone ID: OTI1H6]

CF501887
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityCanine, Human, Mouse, Rat
TargetSOD2
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    SOD2 Mouse Monoclonal Antibody [Clone ID: OTI1H6]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1H6
  • Gene ID6648
  • Target name
    SOD2
  • Target description
    superoxide dismutase 2
  • Target synonyms
    GC1, GClnc1, IPO-B, IPOB, MNSOD, MVCD6, Mn-SOD, lncRNA-GC1, superoxide dismutase [Mn], mitochondrial, Mn superoxide dismutase, epididymis secretory sperm binding protein, gastric cancer-associated lncRNA 1, indophenoloxidase B, manganese-containing superoxide dismutase, mangano-superoxide dismutase, superoxide dismutase 2, mitochondrial
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP04179
  • Protein Name
    Superoxide dismutase [Mn], mitochondrial
  • Scientific Description
    Carrier-free (BSA/glycerol-free) SOD2 mouse monoclonal antibody, clone OTI1H6 (formerly 1H6)
  • Reactivity
    Canine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of SOD2 using anti-SOD2 antibody (PB9442). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: Human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOD2 antigen affinity purified polyclonal antibody (Catalog # PB9442) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOD2 at approximately 24 kDa. The expected band size for SOD2 is at 25 kDa.
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