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ProductsBack/Sorbitol Dehydrogenase antibody [1D5]
The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099
The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099
The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099

Sorbitol Dehydrogenase antibody [1D5]

GTX83588
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSORD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Sorbitol Dehydrogenase antibody [1D5]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1D5
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6652
  • Target name
    SORD
  • Target description
    sorbitol dehydrogenase
  • Target synonyms
    HEL-S-95n, HMNR8, RDH, SDH, SORD1, SORDD, XDH, sorbitol dehydrogenase, (R,R)-butanediol dehydrogenase, L-iditol 2-dehydrogenase, epididymis secretory sperm binding protein Li 95n, polyol dehydrogenase, ribitol dehydrogenase, xylitol dehydrogenase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ00796
  • Protein Name
    Sorbitol dehydrogenase
  • Scientific Description
    Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • García-Arroyo FE, Tapia E, Muñoz-Jiménez I, et al. Fluid Intake Restriction Concomitant to Sweetened Beverages Hydration Induce Kidney Damage. Oxid Med Cell Longev. 2020,2020:8850266. doi: 10.1155/2020/8850266
    Read this paper
  • García-Arroyo FE, Muñoz-Jiménez I, Gonzaga G, et al. A Role for Both V1a and V2 Receptors in Renal Heat Stress Injury Amplified by Rehydration with Fructose. Int J Mol Sci. 2019,20(22). doi: 10.3390/ijms20225764
    Read this paper

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Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (A07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # A07851-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.
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