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ProductsBack/Sorbitol Dehydrogenase antibody [9E7]
WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83589 Sorbitol Dehydrogenase antibody [9E7]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83589 Sorbitol Dehydrogenase antibody [9E7]. Loading : 5 ug per lane
WB analysis of HEK293T cells transfected with Sorbitol Dehydrogenase plasmid (Right) or empty vector (Left) for 48 hrs using GTX83589 Sorbitol Dehydrogenase antibody [9E7]. Loading : 5 ug per lane

Sorbitol Dehydrogenase antibody [9E7]

GTX83589
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetSORD
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Overview

  • Supplier
    GeneTex
  • Product Name
    Sorbitol Dehydrogenase antibody [9E7] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    0.9 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6652
  • Target name
    SORD
  • Target description
    sorbitol dehydrogenase
  • Target synonyms
    (R,R)-butanediol dehydrogenase; epididymis secretory sperm binding protein Li 95n; HEL-S-95n; L-iditol 2-dehydrogenase; polyol dehydrogenase; RDH; ribitol dehydrogenase; SDH; sorbitol dehydrogenase; SORD1; SORDD; XDH; xylitol dehydrogenase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ00796
  • Protein Name
    Sorbitol dehydrogenase
  • Scientific Description
    Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (A07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # A07851-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.
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IP analysis of DDDDK tagged Sorbitol Dehydrogenase overexpressed HEK293T lysate using GTX83583 Sorbitol Dehydrogenase antibody [2A10].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate
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The data was published in the journal Int J Mol Sci in 2019.PMID: 31744099
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