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ProductsBack/SORD antibody [AT10F4]
ICC/IF analysis of Hep3B cells using GTX57595 SORD antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of Hep3B cells using GTX57595 SORD antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of Hep3B cells using GTX57595 SORD antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100

SORD antibody [AT10F4]

GTX57595
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetSORD
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Overview

  • Supplier
    GeneTex
  • Product Name
    SORD antibody [AT10F4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: Recommended starting dilution is 1:1000.. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT10F4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6652
  • Target name
    SORD
  • Target description
    sorbitol dehydrogenase
  • Target synonyms
    (R,R)-butanediol dehydrogenase; epididymis secretory sperm binding protein Li 95n; HEL-S-95n; L-iditol 2-dehydrogenase; polyol dehydrogenase; RDH; ribitol dehydrogenase; SDH; sorbitol dehydrogenase; SORD1; SORDD; XDH; xylitol dehydrogenase
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00796
  • Protein Name
    Sorbitol dehydrogenase
  • Scientific Description
    Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Sorbitol Dehydrogenase/ SORD using anti-Sorbitol Dehydrogenase/ SORD antibody (A07851-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U937 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: monkey kidney tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse liver tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sorbitol Dehydrogenase/ SORD antigen affinity purified polyclonal antibody (Catalog # A07851-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/ SORD at approximately 43KD. The expected band size for Sorbitol Dehydrogenase/ SORD is at 38KD.
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