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ProductsBack/SOX2 antibody [GT1876]
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE GTX627404 diluted at 1:5000
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE GTX627404 diluted at 1:5000
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG D: SK-N-SH 10% SDS PAGE GTX627404 diluted at 1:5000

SOX2 antibody [GT1876]

GTX627404
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat, Zebra Fish
TargetSOX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    SOX2 antibody [GT1876]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. IHC-Fr: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1876
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6657
  • Target name
    SOX2
  • Target description
    SRY-box transcription factor 2
  • Target synonyms
    ANOP3, MCOPS3, transcription factor SOX-2, SRY (sex determining region Y)-box 2, SRY-box 2, SRY-related HMG-box gene 2, sex determining region Y-box 2, transcription factor SOX2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP48431
  • Protein Name
    Transcription factor SOX-2
  • Scientific Description
    This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat, Zebra Fish
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Mashiko T, Kanayama K, Saito N, et al. Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres. Cells. 2021,10(3). doi: 10.3390/cells10030560
    Read this paper
  • Lee KY, Kuo TC, Chou CM, et al. Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling. Cells. 2020,10(1). doi: 10.3390/cells10010028
    Read this paper
  • Zhang L, Liu F, Weygant N, et al. A novel integrated system using patient-derived glioma cerebral organoids and xenografts for disease modeling and drug screening. Cancer Lett. 2021,500:87-97. doi: 10.1016/j.canlet.2020.12.013
    Read this paper
  • Ma L, Lou S, Miao Z, et al. Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate. J Cell Mol Med. 2020,24(23):13669-13678. doi: 10.1111/jcmm.15878
    Read this paper
  • Andreucci E, Peppicelli S, Ruzzolini J, et al. The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells. J Mol Med (Berl). 2020,98(10):1431-1446. doi: 10.1007/s00109-020-01959-y
    Read this paper
  • Aminuddin A, Ng PY, Leong CO, et al. Mitochondrial DNA alterations may influence the cisplatin responsiveness of oral squamous cell carcinoma. Sci Rep. 2020,10(1):7885. doi: 10.1038/s41598-020-64664-3
    Read this paper
  • Peng L, Zhou Y, Xu W, et al. Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts. Int J Biol Sci. 2019,15(11):2340-2349. doi: 10.7150/ijbs.34010
    Read this paper
  • Lin YH, Wu MH, Huang YH, et al. Thyroid hormone negatively regulates tumorigenesis through suppression of BC200. Endocr Relat Cancer. 2018,25(12):967-979. doi: 10.1530/ERC-18-0176
    Read this paper
  • Chuang HM, Ho LI, Huang MH, et al. Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide. Int J Mol Sci. 2018,19(10). doi: 10.3390/ijms19103024
    Read this paper
  • Chang KY, Huang CT, Hsu TI, et al. Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma. Biochem Biophys Res Commun. 2017,493(1):14-19. doi: 10.1016/j.bbrc.2017.09.095
    Read this paper

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Figure 1. Western blot analysis of SOX2 using anti-SOX2 antibody (A00105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat PC-12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 ma for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX2 antigen affinity purified poly clonal antibody (Catalog # A00105-1) at 0.5 microg/ml overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit ING-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Luminescence detection (ECL) kit (Catalog # EK1002) with Anon 5200 system. A specific band was detected for SOX2 at approximately 36 kDa. The expected band size for SOX2 is at 34 kDa.
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