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Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA818674ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA818674ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA818674ESR1HU at dilution of 1:100

SPAG16 Antibody

CSB-PA818674ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSPAG16
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPAG16 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79582
  • Target name
    SPAG16
  • Target description
    sperm associated antigen 16
  • Target synonyms
    PF20; pf20 protein homolog; sperm-associated antigen 16 protein; sperm-associated WD repeat protein; WD repeat domain 29; WDR29
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N0X2
  • Protein Name
    Sperm-associated antigen 16 protein
  • Scientific Description
    Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia .
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPAG16 using anti-SPAG16 antibody (A09080-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG16 antigen affinity purified polyclonal antibody (Catalog # A09080-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAG16 at approximately 71 kDa. The expected band size for SPAG16 is at 71 kDa.
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