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SPAG16 Antibody

LS-C349176
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSPAG16
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPAG16 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 20kDa/29kDa/39kDa/50kDa/70kDa, while the observed MW by Western blot was 71kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 20kDa/29kDa/39kDa/50kDa/70kDa, while the observed MW by Western blot was 71kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID79582
  • Target name
    SPAG16
  • Target description
    sperm associated antigen 16
  • Target synonyms
    PF20; pf20 protein homolog; sperm-associated antigen 16 protein; sperm-associated WD repeat protein; WD repeat domain 29; WDR29
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPAG16 using anti-SPAG16 antibody (A09080-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human RT4 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Raw264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPAG16 antigen affinity purified polyclonal antibody (Catalog # A09080-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPAG16 at approximately 71 kDa. The expected band size for SPAG16 is at 71 kDa.
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