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Western Blot analysis of A375 cell using SPARC Polyclonal Antibody at dilution of 1:400
Western Blot analysis of A375 cell using SPARC Polyclonal Antibody at dilution of 1:400
Western Blot analysis of A375 cell using SPARC Polyclonal Antibody at dilution of 1:400

SPARC Polyclonal Antibody

E-AB-10612
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetSPARC
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Overview

  • Supplier
    Elabscience
  • Product Name
    SPARC Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6678
  • Target name
    SPARC
  • Target description
    secreted protein acidic and cysteine rich
  • Target synonyms
    BM-40, OI17, ON, ONT, SPARC, basement-membrane protein 40, secreted protein, acidic, cysteine-rich (osteonectin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09486
  • Protein Name
    SPARC
  • Scientific Description
    This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A375 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
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