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SPG20 / SPARTIN Antibody (aa500-550)

LS-C762890
LifeSpan BioSciences
ApplicationsImmunoPrecipitation
Product group Antibodies
TargetSPART
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SPG20 / SPARTIN Antibody (aa500-550)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation
  • Applications Supplier
    IP Immunoprecipitation Range: 2-10 µg/mg lysate WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23111
  • Target name
    SPART
  • Target description
    spartin
  • Target synonyms
    SPG20, TAHCCP1, spartin, spastic paraplegia 20 (Troyer syndrome), trans-activated by hepatitis C virus core protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPART using anti-SPART antibody (A31948-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPART antigen affinity purified polyclonal antibody (Catalog # A31948-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPART at approximately 78 kDa. The expected band size for SPART is at 73 kDa.
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