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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022601LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022601LA01HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA022601LA01HU at dilution of 1:100

SPOP Antibody

CSB-PA022601LA01HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetSPOP
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Overview

  • Supplier
    Cusabio
  • Product Name
    SPOP Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8405
  • Target name
    SPOP
  • Target description
    speckle type BTB/POZ protein
  • Target synonyms
    BTBD32; HIB homolog 1; NEDMACE; NEDMIDF; NSDVS1; NSDVS2; roadkill homolog 1; speckle-type POZ protein; TEF2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43791
  • Protein Name
    Speckle-type POZ protein
  • Scientific Description
    Component of a cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex that mediates the ubiquitination of target proteins, leading most often to their proteasomal degradation. In complex with CUL3, involved in ubiquitination and proteasomal degradation of BRMS1, DAXX, PDX1/IPF1, GLI2 and GLI3. In complex with CUL3, involved in ubiquitination of H2AFY and BMI1; this does not lead to their proteasomal degradation. Inhibits transcriptional activation of PDX1/IPF1 targets, such as insulin, by promoting PDX1/IPF1 degradation. The cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex containing homodimeric SPOP has higher ubiquitin ligase activity than the complex that contains the heterodimer formed by SPOP and SPOPL.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPOP using anti-SPOP antibody (A02032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOP antigen affinity purified polyclonal antibody (Catalog # A02032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOP at approximately 50 kDa. The expected band size for SPOP is at 42 kDa.
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