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ProductsBack/SPOP antibody [N1C1]
Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX106942 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX106942 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Sample (50 μg of whole cell lysate) A: mouse brain 10% SDS PAGE GTX106942 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SPOP antibody [N1C1]

GTX106942
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetSPOP
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Overview

  • Supplier
    GeneTex
  • Product Name
    SPOP antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8405
  • Target name
    SPOP
  • Target description
    speckle type BTB/POZ protein
  • Target synonyms
    BTBD32, NEDMACE, NEDMIDF, NSDVS1, NSDVS2, TEF2, speckle-type POZ protein, HIB homolog 1, roadkill homolog 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43791
  • Protein Name
    Speckle-type POZ protein
  • Scientific Description
    This gene encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • He Y, Chen J, Peng X, et al. Clinicopathological and prognostic significance of speckle-type POZ protein in cancers: a systematic review and meta-analysis. Cancer Cell Int. 2021,21(1):626. doi: 10.1186/s12935-021-02329-5
    Read this paper
  • Luo J, Chen B, Gao CX, et al. SPOP promotes FADD degradation and inhibits NF-κB activity in non-small cell lung cancer. Biochem Biophys Res Commun. 2018,504(1):289-294. doi: 10.1016/j.bbrc.2018.08.176
    Read this paper
  • Luo J, Bao YC, Ji XX, et al. SPOP promotes SIRT2 degradation and suppresses non-small cell lung cancer cell growth. Biochem Biophys Res Commun. 2017,483(2):880-884. doi: 10.1016/j.bbrc.2017.01.027
    Read this paper
  • Kim MS, Je EM, Oh JE, et al. Mutational and expressional analyses of SPOP, a candidate tumor suppressor gene, in prostate, gastric and colorectal cancers. APMIS. 2013,121(7):626-33. doi: 10.1111/apm.12030
    Read this paper

Datasheet

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Figure 1. Western blot analysis of SPOP using anti-SPOP antibody (A02032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOP antigen affinity purified polyclonal antibody (Catalog # A02032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOP at approximately 50 kDa. The expected band size for SPOP is at 42 kDa.
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