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SPOP Antibody (YA2054, Rabbit)

HY-P82309
MedChem Express
TargetSPOP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SPOP Antibody (YA2054, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID8405
  • Target name
    SPOP
  • Target description
    speckle type BTB/POZ protein
  • Target synonyms
    BTBD32; HIB homolog 1; NEDMACE; NEDMIDF; NSDVS1; NSDVS2; roadkill homolog 1; speckle-type POZ protein; TEF2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SPOP Antibody (YA2054) is a non-conjugated IgG antibody, targeting SPOP, with a predicted molecular weight of 42 kDa (observed band size: 42 kDa). SPOP Antibody (YA2054) can be used for WB experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPOP using anti-SPOP antibody (A02032-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde r reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPOP antigen affinity purified polyclonal antibody (Catalog # A02032-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPOP at approximately 50 kDa. The expected band size for SPOP is at 42 kDa.
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