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WB analysis of wild type and SPR knock out HeLa cell lysate(10microg per lane) using GTX83572 SPR antibody [1F4]. Dilution : 1:500
WB analysis of wild type and SPR knock out HeLa cell lysate(10microg per lane) using GTX83572 SPR antibody [1F4]. Dilution : 1:500
WB analysis of wild type and SPR knock out HeLa cell lysate(10microg per lane) using GTX83572 SPR antibody [1F4]. Dilution : 1:500

SPR antibody [1F4]

GTX83572
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetSPR
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Overview

  • Supplier
    GeneTex
  • Product Name
    SPR antibody [1F4]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100. FACS: 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F4
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6697
  • Target name
    SPR
  • Target description
    sepiapterin reductase
  • Target synonyms
    SDR38C1, sepiapterin reductase, Sepiapterin reductase (L-erythro-7,8-dihydrobiopterin forming), sepiapterin reductase (7,8-dihydrobiopterin:NADP+ oxidoreductase), short chain dehydrogenase/reductase family 38C, member 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP35270
  • Protein Name
    Sepiapterin reductase
  • Scientific Description
    Catalyzes the final one or two reductions in tetra-hydrobiopterin biosynthesis to form 5,6,7,8-tetrahydrobiopterin.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPR using anti-SPR antibody (A00416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPR antigen affinity purified polyclonal antibody (Catalog # A00416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPR at approximately 28KD. The expected band size for SPR is at 28KD.
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