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Western Blot analysis of Mouse liver and Human chromaffin cell tumor tissue, hepG2 cell using SPR Polyclonal Antibody at dilution of 1:1500
Western Blot analysis of Mouse liver and Human chromaffin cell tumor tissue, hepG2 cell using SPR Polyclonal Antibody at dilution of 1:1500
Western Blot analysis of Mouse liver and Human chromaffin cell tumor tissue, hepG2 cell using SPR Polyclonal Antibody at dilution of 1:1500

SPR Polyclonal Antibody

E-AB-16217
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    SPR Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPR using anti-SPR antibody (A00416-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPR antigen affinity purified polyclonal antibody (Catalog # A00416-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPR at approximately 28KD. The expected band size for SPR is at 28KD.
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The image on the left is immunohistochemistry of paraffin-embedded Human breast cancer tissue using CSB-PA214577(SPR Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
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SPR antibody [N2C3] immunoprecipitates SPR protein in IP experiments. IP samples: A431 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of SPR protein by 4 μg SPR antibody [N2C3] (GTX113552) 5 % SDS-PAGE The immunoprecipitated SPR protein was detected by SPR antibody [N2C3] (GTX113552) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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