Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 microg/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.
Immunohistochemistry of Rabbit anti-Sprouty-4 antibody. Tissue: human liver tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required.Primary antibody: Sprouty-4 antibody at 2.5 microg/mL for 1 h at RT.Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT.Localization: Sprouty-4 is cytoplasmic.Staining: Sprouty-4 as precipitated brown with blue nuclear counterstain.
SPRY4 antibody
GTX20265
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetSPRY4
Overview
- SupplierGeneTex
- Product NameSPRY4 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:100-1:1000. IHC-P: 1-10 microg/mL. ELISA: 1:3000-1:12000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.1 mg/ml
- ConjugateUnconjugated
- Gene ID81848
- Target nameSPRY4
- Target descriptionsprouty RTK signaling antagonist 4
- Target synonymsHH17; protein sprouty homolog 4; sprouty homolog 4
- HostRabbit
- IsotypeIgG
- Protein IDQ9C004
- Protein NameProtein sprouty homolog 4
- Scientific DescriptionSprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203