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Western Blot analysis of 293 cells using c-Src Polyclonal Antibody
Western Blot analysis of 293 cells using c-Src Polyclonal Antibody
Western Blot analysis of 293 cells using c-Src Polyclonal Antibody

SRC Antibody

CSB-PA001803
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSRC
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Overview

  • Supplier
    Cusabio
  • Product Name
    SRC Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6714
  • Target name
    SRC
  • Target description
    SRC proto-oncogene, non-receptor tyrosine kinase
  • Target synonyms
    ASV; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; proto-oncogene tyrosine-protein kinase Src; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12931
  • Protein Name
    Proto-oncogene tyrosine-protein kinase Src
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
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Figure 1. Western blot analysis of Src using anti-Src antibody (PB9059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Src antigen affinity purified polyclonal antibody (Catalog # PB9059) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Src at approximately 60 kDa. The expected band size for Src is at 60 kDa.
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