
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (GTX133473) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Src (phospho Tyr530) antibody
GTX133473
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSRC
Overview
- SupplierGeneTex
- Product NameSrc (phospho Tyr530) antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.18 mg/ml
- ConjugateUnconjugated
- Gene ID6714
- Target nameSRC
- Target descriptionSRC proto-oncogene, non-receptor tyrosine kinase
- Target synonymsASV, SRC1, THC6, c-SRC, p60-Src, proto-oncogene tyrosine-protein kinase Src, proto-oncogene c-Src, protooncogene SRC, Rous sarcoma, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
- HostRabbit
- IsotypeIgG
- Protein IDP12931
- Protein NameProto-oncogene tyrosine-protein kinase Src
- Scientific DescriptionThis gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161







![Western blot analysis on A431 cell lysates using Src (phospho Tyr416) antibody [EPR6239(2)] (GTX63902). Cells were either (A) untreated or (B) treated with Pervanadate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX63902/GTX63902_WB_w_23061202_568.webp)
