More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/SRC Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with SRC Polyclonal Antibody, Unconjugated (bs-1135R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.

SRC Polyclonal Antibody

BS-1135R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSRC
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    SRC Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6714
  • Target name
    SRC
  • Target description
    SRC proto-oncogene, non-receptor tyrosine kinase
  • Target synonyms
    ASV; c-SRC; p60-Src; proto-oncogene c-Src; protooncogene SRC, Rous sarcoma; proto-oncogene tyrosine-protein kinase Src; SRC1; THC6; tyrosine kinase pp60c-src; tyrosine-protein kinase SRC-1; v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP12931
  • Protein Name
    Proto-oncogene tyrosine-protein kinase Src
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
SRC Polyclonal AntibodyCAC15899
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetSRC
  • SizePrice
Product group Antibodies
Assay Genie
Src AntibodyCAB0324
TargetSRC
  • SizePrice
Product group Antibodies
MedChem Express
Src AntibodyHY-P80338
TargetSRC
  • SizePrice
Western Blot analysis of 293 cells using c-Src Polyclonal Antibody
Product group Antibodies
Cusabio
SRC AntibodyCSB-PA001803
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetSRC
  • SizePrice
Product group Antibodies
References
GeneTex
Src (phospho Tyr530) antibodyGTX133473
ApplicationsWestern Blot
TargetSRC
  • SizePrice
Anti-Src Antibody
Product group Antibodies
Antibodies.com
Anti-Src AntibodyA96431
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
RayBiotech
Anti-SRC Antibody144-61603
ApplicationsImmunoPrecipitation, Western Blot
TargetSRC
  • SizePrice
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
Product group Antibodies
Atlas Antibodies
Anti-SRC AntibodyHPA030875
ApplicationsWestern Blot, ImmunoCytoChemistry
TargetSRC
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
SRC AntibodyLS-C831467
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetSRC
  • SizePrice
Figure 1. Western blot analysis of Src using anti-Src antibody (PB9059). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Src antigen affinity purified polyclonal antibody (Catalog # PB9059) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Src at approximately 60 kDa. The expected band size for Src is at 60 kDa.
Product group Antibodies
Boster Bio
Anti-Src Antibody Picoband(r)PB9059-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetSRC
  • SizePrice