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Western Blot analysis of HuvEc cells using SSH3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using SSH3 Polyclonal Antibody
Western Blot analysis of HuvEc cells using SSH3 Polyclonal Antibody

SSH3 Antibody

CSB-PA004163
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSSH3
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Overview

  • Supplier
    Cusabio
  • Product Name
    SSH3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54961
  • Target name
    SSH3
  • Target description
    slingshot protein phosphatase 3
  • Target synonyms
    hSSH-3L; protein phosphatase Slingshot homolog 3; slingshot 3; slingshot homolog 3; SSH3L; SSH-3L; SSH-like protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TE77
  • Protein Name
    Protein phosphatase Slingshot homolog 3
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSH3 using anti-SSH3 antibody (A11609-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293Twhole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3 antigen affinity purified polyclonal antibody (Catalog # A11609-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3 at approximately 95 kDa. The expected band size for SSH3 is at 73 kDa.
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