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SSH3 Polyclonal Antibody

CAC15325
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetSSH3
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Overview

  • Supplier
    Biomatik
  • Product Name
    SSH3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54961
  • Target name
    SSH3
  • Target description
    slingshot protein phosphatase 3
  • Target synonyms
    SSH3L, protein phosphatase Slingshot homolog 3, SSH-3L, SSH-like protein 3, hSSH-3L, slingshot 3, slingshot homolog 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TE77
  • Protein Name
    Protein phosphatase Slingshot homolog 3
  • Scientific Description
    The SSH3 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSH3 using anti-SSH3 antibody (A11609-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293Twhole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3 antigen affinity purified polyclonal antibody (Catalog # A11609-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSH3 at approximately 95 kDa. The expected band size for SSH3 is at 73 kDa.
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