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SSSCA1 Polyclonal Antibody

RD218934A
Reddot Biotech
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetZNRD2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    SSSCA1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.84 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10534
  • Target name
    ZNRD2
  • Target description
    zinc ribbon domain containing 2
  • Target synonyms
    autoantigen p27; centromeric autoantigen (27kD); p27; protein ZNRD2; sjoegren syndrome/scleroderma autoantigen 1; Sjogren syndrome/scleroderma autoantigen 1; Sjogren's syndrome/scleroderma autoantigen 1; SSSCA1; zinc ribbon domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This antigen is recognized by a subset of anti-centromere antibodies from patients with scleroderma and/or Sjogrens syndrome. Subcellular localization has not yet been established.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SSSCA1/ZNRD2 using anti-SSSCA1/ZNRD2 antibody (A14259-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: rat ovary tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSSCA1/ZNRD2 antigen affinity purified polyclonal antibody (Catalog # A14259-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSSCA1/ZNRD2 at approximately 23 kDa. The expected band size for SSSCA1/ZNRD2 is at 21 kDa.
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