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ST6GAL1 / CD75 Antibody

LS-C813936
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetST6GAL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ST6GAL1 / CD75 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6480
  • Target name
    ST6GAL1
  • Target description
    ST6 beta-galactoside alpha-2,6-sialyltransferase 1
  • Target synonyms
    CDw75, SIAT1, ST6GalI, ST6N, beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, CMP-N-acetylneuraminate beta-galactosamide alpha-2,6-sialyltransferase, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, ST6 N-acetylgalactosaminide alpha-2,6-sialyltransferase 1, ST6 beta-galactosamide alpha-2,6-sialyltranferase 1, alpha 2,6-ST 1, sialyltransferase 1 (beta-galactoside alpha-2,6-sialyltransferase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of CDw75/ST6GAL1 using anti-CDw75/ST6GAL1 antibody (A03156-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDw75/ST6GAL1 antigen affinity purified polyclonal antibody (Catalog # A03156-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDw75/ST6GAL1 at approximately 47 kDa. The expected band size for CDw75/ST6GAL1 is at 47 kDa.
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