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Mouse brain lysates probed with Stat2 (Tyr690) Polyclonal Antibody, unconjugated (bs-3428R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse brain lysates probed with Stat2 (Tyr690) Polyclonal Antibody, unconjugated (bs-3428R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Mouse brain lysates probed with Stat2 (Tyr690) Polyclonal Antibody, unconjugated (bs-3428R) at 1:300 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

Phospho-Stat2 (Tyr690) Polyclonal Antibody

BS-3428R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Rat
TargetSTAT2
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Overview

  • Supplier
    Bioss
  • Product Name
    Phospho-Stat2 (Tyr690) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6773
  • Target name
    STAT2
  • Target description
    signal transducer and activator of transcription 2
  • Target synonyms
    IMD44, ISGF-3, P113, PTORCH3, STAT113, signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator, signal transducer and activator of transcription 2, 113kDa
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52630
  • Protein Name
    Signal transducer and activator of transcription 2
  • Reactivity
    Canine, Equine, Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Alves de Medeiros AK, Speeckaert R, Desmet E, et al. JAK3 as an Emerging Target for Topical Treatment of Inflammatory Skin Diseases. PLoS One. 2016,11(10):e0164080. doi: 10.1371/journal.pone.0164080
    Read this paper

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Figure 1. Western blot analysis of STAT2 using anti-STAT2 antibody (A01360-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HT-1080 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT2 antigen affinity purified polyclonal antibody (Catalog # A01360-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for STAT2 at approximately 113 kDa. The expected band size for STAT2 is at 98 kDa.
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