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ProductsBack/STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT3 (Cat# RC212394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3(Cat# TA500177). Positive lysates [LY403728] (100ug) and [LC403728] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT3 (Cat# RC212394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3(Cat# TA500177). Positive lysates [LY403728] (100ug) and [LC403728] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY STAT3 (Cat# RC212394, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-STAT3(Cat# TA500177). Positive lysates [LY403728] (100ug) and [LC403728] (20ug) can be purchased separately from OriGene.

STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]

CF500177
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTAT3
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    STAT3 Mouse Monoclonal Antibody [Clone ID: OTI21E7]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI21E7
  • Gene ID6774
  • Target name
    STAT3
  • Target description
    signal transducer and activator of transcription 3
  • Target synonyms
    ADMIO, ADMIO1, APRF, HIES, signal transducer and activator of transcription 3, DNA-binding protein APRF, acute-phase response factor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP40763
  • Protein Name
    Signal transducer and activator of transcription 3
  • Scientific Description
    Carrier-free (BSA/glycerol-free) STAT3 mouse monoclonal antibody, clone OTI21E7 (formerly 21E7)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of STAT3 using anti-STAT3 antibody (PB9511). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: human K562 whole cell lysates, Lane 6: human SiHa whole cell lysates, Lane 7: human RT4 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT3 antigen affinity purified polyclonal antibody (Catalog # PB9511) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for STAT3 at approximately 88 kDa. The expected band size for STAT3 is at 88 kDa.
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