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Steroid sulfatase antibody detects Steroid sulfatase protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Steroid sulfatase antibody (GTX113628) dilution: 1:1000
Steroid sulfatase antibody detects Steroid sulfatase protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Steroid sulfatase antibody (GTX113628) dilution: 1:1000
Steroid sulfatase antibody detects Steroid sulfatase protein by western blot analysis. A. 30 μg Huh7 whole cell lysate/extract B. 30 μg Hep3B whole cell lysate/extract C. 30 μg HepG2 whole cell lysate/extract 7.5 % SDS-PAGE Steroid sulfatase antibody (GTX113628) dilution: 1:1000

Steroid sulfatase antibody

GTX113628
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetSTS
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Overview

  • Supplier
    GeneTex
  • Product Name
    Steroid sulfatase antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID412
  • Target name
    STS
  • Target description
    steroid sulfatase
  • Target synonyms
    ARSC, ARSC1, ASC, ES, SSDD, XLI, steryl-sulfatase, arylsulfatase C, estrone sulfatase, steroid sulfatase (microsomal), isozyme S, steryl-sulfate sulfohydrolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08842
  • Protein Name
    Steryl-sulfatase
  • Scientific Description
    The protein encoded by this gene catalyzes the conversion of sulfated steroid precursors to estrogens during pregnancy. The encoded protein is found in the endoplasmic reticulum, where it acts as a homodimer. Mutations in this gene are known to cause X-linked ichthyosis (XLI). [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Steroid sulfatase using anti-Steroid sulfatase antibody (A01198-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human PANC-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Steroid sulfatase antigen affinity purified polyclonal antibody (Catalog # A01198-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Steroid sulfatase at approximately 65KD. The expected band size for Steroid sulfatase is at 65KD.
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