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SUCLG2 Antibody

LS-C410510
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetSUCLG2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SUCLG2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 46kDa/47kDa, while the observed MW by Western blot was 47kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:20 - 1:200), WB (1:500 - 1:2000) The predicted MW is 46kDa/47kDa, while the observed MW by Western blot was 47kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8801
  • Target name
    SUCLG2
  • Target description
    succinate-CoA ligase GDP-forming subunit beta
  • Target synonyms
    GBETA; G-SCS; GTPSCS; GTP-specific succinyl-CoA synthetase beta subunit; GTP-specific succinyl-CoA synthetase subunit beta; SCS-betaG; succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial; succinate-CoA ligase GDP-forming beta subunit; succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial; succinyl-CoA ligase, GDP-forming, beta chain, mitochondrial; succinyl-CoA synthetase, beta-G chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SUCLG2 using anti-SUCLG2 antibody (A08268-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human COLO320 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUCLG2 antigen affinity purified polyclonal antibody (Catalog # A08268-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUCLG2 at approximately 47 kDa. The expected band size for SUCLG2 is at 47 kDa.
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Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, 293T whole cell lysate, Raji whole cell lysate, LO2 whole cell lysate, U87 whole cell lysate, All lanes: SUCLG2 antibody at 6.7microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 48 kDa Observed band size: 47 kDa
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