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SULT2B1 Polyclonal Antibody

RD76132A
Reddot Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSULT2B1
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    SULT2B1 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6820
  • Target name
    SULT2B1
  • Target description
    sulfotransferase family 2B member 1
  • Target synonyms
    ARCI14, HSST2, sulfotransferase 2B1, ST2B1, alcohol sulfotransferase, hydroxysteroid sulfotransferase 2, hydroxysteroid sulfotransferase SULT2B1a, sulfotransferase family, cytosolic, 2B, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene sulfates dehydroepiandrosterone but not 4-nitrophenol, a typical substrate for the phenol and estrogen sulfotransferase subfamilies. Two alternatively spliced variants that encode different isoforms have been described.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: A431 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.
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