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ProductsBack/SULT2B1 / Sulfotransferase 2B1 Antibody (aa144-193)

SULT2B1 / Sulfotransferase 2B1 Antibody (aa144-193)

LS-C81269
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetSULT2B1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    SULT2B1 / Sulfotransferase 2B1 Antibody (aa144-193)
  • Delivery Days Customer
    14
  • Application Supplier Note
    ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Applications
    Western Blot
  • Applications Supplier
    WB ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6820
  • Target name
    SULT2B1
  • Target description
    sulfotransferase family 2B member 1
  • Target synonyms
    alcohol sulfotransferase; ARCI14; HSST2; hydroxysteroid sulfotransferase 2; hydroxysteroid sulfotransferase SULT2B1a; ST2B1; sulfotransferase 2B1; sulfotransferase family, cytosolic, 2B, member 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of SULT2B1 using anti-SULT2B1 antibody (PB9822). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Testis Tissue Lysate at 50ug, Lane 2: A431 Whole Cell Lysate at 40ug, Lane 3: HELA Whole Cell Lysate at 40ug, Lane 4: MCF-7 Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SULT2B1 antigen affinity purified polyclonal antibody (Catalog # PB9822) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SULT2B1 at approximately 48 kDa. The expected band size for SULT2B1 is at 48 kDa.
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