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ProductsBack/Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded human gastric carcinoma labeled with Rabbit Anti Phospho-Syk (Tyr525/526) Polyclonal Antibody, Unconjugated (bs-3434R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Phospho-Syk (Tyr525 + Tyr526) Polyclonal Antibody

BS-3434R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSYK
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Syk (Tyr525 + Tyr526) Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID6850
  • Target name
    SYK
  • Target description
    spleen associated tyrosine kinase
  • Target synonyms
    IMD82; p72-Syk; spleen tyrosine kinase; tyrosine-protein kinase SYK
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43405
  • Protein Name
    Tyrosine-protein kinase SYK
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Berberine suppresses mast cell-mediated allergic responses via regulating FcepsilonRI-mediated and MAPK signaling. Fu S et al., 2019 Jun, Int Immunopharmacol
    Read more

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Figure 1. Flow Cytometry analysis of A431 cells using anti-SYK antibody (A00490-3). Overlay histogram showing A431 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYK Antibody (A00490-3,1microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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