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ProductsBack/Synaptophysin antibody [SYP02]
IHC-P analysis of human pancreas tissue using GTX26245 Synaptophysin antibody [SYP02].
IHC-P analysis of human pancreas tissue using GTX26245 Synaptophysin antibody [SYP02].
IHC-P analysis of human pancreas tissue using GTX26245 Synaptophysin antibody [SYP02].

Synaptophysin antibody [SYP02]

GTX26245
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetSYP
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Overview

  • Supplier
    GeneTex
  • Product Name
    Synaptophysin antibody [SYP02]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1:25-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SYP02
  • Conjugate
    Unconjugated
  • Gene ID6855
  • Target name
    SYP
  • Target description
    synaptophysin
  • Target synonyms
    major synaptic vesicle protein P38; MRX96; MRXSYP; synaptophysin; XLID96
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08247
  • Protein Name
    Synaptophysin
  • Scientific Description
    This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with an X-linked form of cognitive disability. [provided by RefSeq, Jul 2017]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Lane 1:Human Cerebrum lysates; Lane 2: PC-12 cell lysates probed with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
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Figure 1. Western blot analysis of SYP using anti-SYP antibody (A05049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP antigen affinity purified polyclonal antibody (Catalog # A05049) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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