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Synaptophysin (SYP) Polyclonal Antibody

CAU31063
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSYP
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Overview

  • Supplier
    Biomatik
  • Product Name
    Synaptophysin (SYP) Polyclonal Anti
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6855
  • Target name
    SYP
  • Target description
    synaptophysin
  • Target synonyms
    major synaptic vesicle protein P38; MRX96; MRXSYP; synaptophysin; XLID96
  • Host
    Rabbit
  • Protein IDP08247
  • Protein Name
    Synaptophysin
  • Scientific Description
    The Synaptophysin (SYP) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SYP using anti-SYP antibody (A05049). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYP antigen affinity purified polyclonal antibody (Catalog # A05049) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYP at approximately 38KD. The expected band size for SYP is at 34KD.
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Lane 1:Human Cerebrum lysates; Lane 2: PC-12 cell lysates probed with Synaptophysin (2B1) Monoclonal Antibody (bsm-52379R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation for 60 min at 37˚C.
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Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA002858 antibody. Corresponding SYP RNA-seq data are presented for the same tissues.
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References
GeneTex
Synaptophysin antibodyGTX100865
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