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TAB1 Antibody

LS-C814133
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetTAB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    TAB1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10454
  • Target name
    TAB1
  • Target description
    TGF-beta activated kinase 1 (MAP3K7) binding protein 1
  • Target synonyms
    3'-Tab1, MAP3K7IP1, TGF-beta-activated kinase 1 and MAP3K7-binding protein 1, TAK1-binding protein 1, mitogen-activated protein kinase kinase kinase 7-interacting protein 1, transforming growth factor beta-activated kinase-binding protein 1
  • Host
    Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TAB1 using anti-TAB1 antibody (A02847-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TAB1 antigen affinity purified polyclonal antibody (Catalog # A02847-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TAB1 at approximately 55-65 kDa. The expected band size for TAB1 is at 55 kDa.
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