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Tag antibodies

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HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150028).
Product group Antibodies
OriGene
Mouse monoclonal turboYFP antibody, clone OTI14C4TA150028
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HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150028).
Product group Antibodies
OriGene
Mouse monoclonal turboYFP antibody, clone OTI14C4TA150028S
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Myc/DDK tagged VARS2 overexpression lysate and pull-down by various batches of anti-DDK beads were detected with HRP-conjugated 4C5 anti-DDK antibody (TA150030).
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal antibody, HRPTA150030
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Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~19 kDa), using His-tag antibody. A: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), non-induced (10 ug). B: 10ug E.coli Lysate harbouring His-tagged recombinant protein (N-terminal linked), induced (10 ug).
Product group Antibodies
OriGene
Anti-6X His tag rabbit polyclonal antibodyTA150031
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Western blot analysis of 1 ug of whole cell lysate with anti-GFP antibody TA150032 diluted at 1:10000. A: Non-transfected 293T lysates; B: GFP transfected 293T lysates; C: RFP transfected 293T lysates.
Product group Antibodies
OriGene
Rabbit polyclonal eGFP antibodyTA150032
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Sample: A: 20ug of E. coli only; B: 20ug of Transformed E. coli; C: 20ug of Transformed E. coli and IPTG induced. 12% SDS PAGE, TA150033 diluted at 1:3000.
Product group Antibodies
OriGene
Anti-GST rabbit polyclonal antibodyTA150033
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Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing HA-tagged protein (~17 kDa) using HA-tag antibody. A: Non-transfected lysate. B: Transfected lysate.
Product group Antibodies
OriGene
Anti-HA tag rabbit polyclonal antibodyTA150034
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Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose BeadsTA150037
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Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
Clone OTI4C5, Anti-DDK (FLAG) Monoclonal Antibody, Agarose BeadsTA150037S
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Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.
Product group Antibodies
OriGene
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic BeadsTA150039
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Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.
Product group Antibodies
OriGene
Mouse Monoclonal tGFP Antibody, Clone OTI2H8, Magnetic BeadsTA150039S
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Immunoblot analysis of randomly picked 7 different tGFP tagged over-expression lysates with 2H8 anti-tGFP monoclonal antibody (TA150041) at 1:2000 dilution.
Product group Antibodies
OriGene
Mouse monoclonal turboGFP antibody, clone OTI2H8TA150041S
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