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Tag antibodies

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Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice
Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoprecipitation was performed with HEK293T cell lysates of expressing Entry vector (control, Cat# PS100001) or expressing DDK-tagged PON3 (Cat# LY400339). Anti-DDK 4C5-agarose beads were incubated with control lysate or LY400339 overnight at 4°C. After washing three times with lysate buffer, 20uL 4x SDS sample buffer was applied. The samples were boiled at 95°C for 10min. The supernatant was loaded to the SDS-PAGE gel after discarded the beads by centrifuging. After transferring to the membrane, the Western blot assay was applied with HRP-conjugated anti-DDK antibody (Cat# TA150030). Lane 1: control lysate; Lane 2: DDK-tagged PON3 (LY400339); Lane 3: anti-DDK 4C5 agarose beads (TA150037) 50uL with control lysate; Lane 4: anti-DDK 4C5 agarose beads (TA150037) 50uL with LY400339.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Immunoprecipitation (IP) was performed with overexpression lysate expressed with tGFP expression vector (Cat# PS100010) or control lysate transfected with pCMV-Entry empty vector (Cat# PS100001). Briefly, 20uL anti-tGFP (2H8) magnetic beads (Cat# TA150039) were incubated with tGFP overexpression lysate or negative control lysate overnight at 4°C, respectively. After washing three times with RIPA buffer, 20uL 4x SDS sample buffer was applied to the lysate-beads mixture. The samples were boiled at 95°C for 10min. The IP sample supernatants were fractionated on SDS-PAGE gel, blotted onto nitrocellulose membrane. The IP efficiency was evaluated by using HRP-conjugated anti-tGFP antibody (Cat# TA150043). Lane 1: anti-tGFP (2H8) magnetic beads (20uL) with tGFP expression lysate; Lane 2: anti-tGFP magnetic beads (20uL) with negative control lysate.
Product group Antibodies
OriGene
ApplicationsImmunoPrecipitation, Purification/Extraction/Isolation
  • SizePrice
Western blot analysis of extracts from HEK293 cells transfected with C-terminal HA-tagged gene (lane 2, 3, 4, with 5, 10, 20uL from left to right) and negative control vector (lane 1) using anti-HA tag rabbit monoclonal antibody (TA591010) at 1:2000 dilution.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Western blot analysis of extracts from HEK293 cells transfected with C-terminal HA-tagged gene (lane 2, 3, 4, with 5, 10, 20uL from left to right) and negative control vector (lane 1) using anti-HA tag rabbit monoclonal antibody (TA591011) at 1:2000 dilution.
Product group Antibodies
OriGene
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
Anti-MBP Ab at 1/2,000 dilution; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetmalE
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Anti-MBP Ab at 1/2,000 dilution; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Product group Antibodies
OriGene
ApplicationsWestern Blot
TargetmalE
  • SizePrice
Anti-GST Ab at 1/2,000 dilution; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution;
Product group Antibodies
OriGene
ApplicationsWestern Blot
  • SizePrice

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