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WB analysis of HepG2 cells using GTX77745 TAL1 antibody at 1.25μg/ml. Lane A : marker Lane B : HepG2 cells
WB analysis of HepG2 cells using GTX77745 TAL1 antibody at 1.25μg/ml. Lane A : marker Lane B : HepG2 cells
WB analysis of HepG2 cells using GTX77745 TAL1 antibody at 1.25μg/ml. Lane A : marker Lane B : HepG2 cells

TAL1 antibody, C-term

GTX77745
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetTAL1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TAL1 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6886
  • Target name
    TAL1
  • Target description
    TAL bHLH transcription factor 1, erythroid differentiation factor
  • Target synonyms
    SCL, TCL5, bHLHa17, tal-1, T-cell acute lymphocytic leukemia protein 1, T-cell acute lymphocytic leukemia 1, T-cell leukemia/lymphoma protein 5, class A basic helix-loop-helix protein 17, stem cell protein, tal-1 product
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP17542
  • Protein Name
    T-cell acute lymphocytic leukemia protein 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Tal1 using anti-Tal1 antibody (A00944-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human U20S cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tal1 antigen affinity purified polyclonal antibody (Catalog # A00944-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tal1 at approximately 39KD. The expected band size for Tal1 is at 34KD.
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