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Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TAX1BP1 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TAX1BP1 Polyclonal Antibody at dilution 1:60
Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TAX1BP1 Polyclonal Antibody at dilution 1:60

TAX1BP1 Polyclonal Antibody

E-AB-10657
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    TAX1BP1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    This gene encodes a HTLV-1 tax1 binding protein. The encoded protein interacts with TNFAIP3, and inhibits TNF-induced apoptosis by mediating the TNFAIP3 anti-apoptotic activity. Degradation of this protein by caspase-3-like family proteins is associated with apoptosis induced by TNF. This protein may also have a role in the inhibition of inflammatory signaling pathways. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TRAF6BP using anti-TRAF6BP antibody (M04336). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human SIHA whole cell lysates, Lane 4: rat testis tissue lysates, Lane 5: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRAF6BP antigen affinity purified monoclonal antibody (Catalog # M04336) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRAF6BP at approximately 91 kDa. The expected band size for TRAF6BP is at 91 kDa.
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References
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