TCP1 epsilon antibody [GT639] detects TCP1 epsilon protein at centrosome and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TCP1 epsilon stained by TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631668/GTX631668_41799_20161110_WB_shRNA_watermark_w_23061202_856.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631668/GTX631668_41799_20190419_WB_TPM_watermark_w_23061202_400.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:1000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [GT639] (GTX631668). Western blot analysis was performed using TCP1 epsilon antibody [GT639] (GTX631668). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX631668/GTX631668_41799_20150604_IP_w_23061202_385.webp "Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 μg of TCP1 epsilon antibody [GT639] (GTX631668). Western blot analysis was performed using TCP1 epsilon antibody [GT639] (GTX631668). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
TCP1 epsilon antibody [GT639] detects TCP1 epsilon protein at centrosome and cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: TCP1 epsilon stained by TCP1 epsilon antibody [GT639] (GTX631668) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 μm.
TCP1 epsilon antibody [GT639]
GTX631668
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHamster, Human
TargetCCT5
Overview
- SupplierGeneTex
- Product NameTCP1 epsilon antibody [GT639]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT639
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID22948
- Target nameCCT5
- Target descriptionchaperonin containing TCP1 subunit 5
- Target synonymsCCT-epsilon, CCTE, HEL-S-69, HSNSP, PNAS-102, TCP-1-epsilon, T-complex protein 1 subunit epsilon, chaperonin containing T-complex polypeptide 1 subunit 5, chaperonin containing TCP1, subunit 5 (epsilon), epididymis secretory protein Li 69
- HostMouse
- IsotypeIgG1
- Protein IDP48643
- Protein NameT-complex protein 1 subunit epsilon
- Scientific DescriptionThe protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Mutations in this gene cause hereditary sensory and autonomic neuropathy with spastic paraplegia (HSNSP). Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 5 and 13. [provided by RefSeq, Apr 2015]
- ReactivityHamster, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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