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Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDG antibody (GTX110473) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

TDG antibody

GTX110473
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetTDG
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Overview

  • Supplier
    GeneTex
  • Product Name
    TDG antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6996
  • Target name
    TDG
  • Target description
    thymine DNA glycosylase
  • Target synonyms
    hTDG, G/T mismatch-specific thymine DNA glycosylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13569
  • Protein Name
    G/T mismatch-specific thymine DNA glycosylase
  • Scientific Description
    The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Liu X, Xu B, Yang J, et al. UHRF2 commissions the completion of DNA demethylation through allosteric activation by 5hmC and K33-linked ubiquitination of XRCC1. Mol Cell. 2021,81(14):2960-2974.e7. doi: 10.1016/j.molcel.2021.05.022
    Read this paper
  • Someda M, Kuroki S, Miyachi H, et al. Caspase-8, receptor-interacting protein kinase 1 (RIPK1), and RIPK3 regulate retinoic acid-induced cell differentiation and necroptosis. Cell Death Differ. 2020,27(5):1539-1553. doi: 10.1038/s41418-019-0434-2
    Read this paper
  • McLaughlin D, Coey CT, Yang WC, et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. 2016,291(17):9014-24. doi: 10.1074/jbc.M115.706325
    Read this paper
  • Jin C, Qin T, Barton MC, et al. Minimal role of base excision repair in TET-induced global DNA demethylation in HEK293T cells. Epigenetics. 2015,10(11):1006-13. doi: 10.1080/15592294.2015.1091145
    Read this paper
  • Moriyama T, Fujimitsu Y, Yoshikai Y, et al. SUMO-modification and elimination of the active DNA demethylation enzyme TDG in cultured human cells. Biochem Biophys Res Commun. 2014,447(3):419-24. doi: 10.1016/j.bbrc.2014.04.004
    Read this paper
  • Xu X, Yu T, Shi J, et al. Thymine DNA glycosylase is a positive regulator of Wnt signaling in colorectal cancer. J Biol Chem. 2014,289(13):8881-90. doi: 10.1074/jbc.M113.538835
    Read this paper

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