HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
![Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (GTX632126). Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632126/GTX632126_42037_20150710_IP_w_23061202_742.webp "Immunoprecipitation of TEF1 protein from HeLa nuclear extracts using 5 μg of TEF1 antibody [GT13112] (GTX632126). Western blot analysis was performed using TEF1 antibody [GT13112] (GTX632126). EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.")
![TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.](https://www.genetex.com/upload/website/prouct_img/normal/GTX632126/GTX632126_42037_20150831_IFA_w_23061202_547.webp "TEF1 antibody [GT13112] detects TEF1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TEF1 protein stained by TEF1 antibody [GT13112] (GTX632126) diluted at 1:400. Red: Phalloidin, a F-actin marker, diluted at 1:200.")
HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TEF1 antibody [GT13112] (GTX632126) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
TEF1 antibody [GT13112]
GTX632126
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetTEAD1
Overview
- SupplierGeneTex
- Product NameTEF1 antibody [GT13112]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT13112
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7003
- Target nameTEAD1
- Target descriptionTEA domain transcription factor 1
- Target synonymsAA, NTEF-1, REF1, TCF-13, TCF13, TEAD-1, TEF-1, transcriptional enhancer factor TEF-1, TEA domain family member 1 (SV40 transcriptional enhancer factor), protein GT-IIC, transcription factor 13, transcriptional enhancer factor 1
- HostMouse
- IsotypeIgG1
- Protein IDP28347
- Protein NameTranscriptional enhancer factor TEF-1
- Scientific DescriptionThis gene encodes a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in this gene cause Sveinssons chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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