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ProductsBack/TET1 antibody [GT1462]
TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
TET1 antibody [GT1462] detects TET1 protein at nucleus on HeLa xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HeLa xenograft. TET1 antibody [GT1462] (GTX627420) dilution: 1:100. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

TET1 antibody [GT1462]

GTX627420
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTET1
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Overview

  • Supplier
    GeneTex
  • Product Name
    TET1 antibody [GT1462]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1462
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80312
  • Target name
    TET1
  • Target description
    tet methylcytosine dioxygenase 1
  • Target synonyms
    CXXC6, LCX, bA119F7.1, methylcytosine dioxygenase TET1, CXXC finger 6, CXXC zinc finger 6, CXXC-type zinc finger protein 6, leukemia-associated protein with a CXXC domain, ten-eleven translocation 1 gene protein, ten-eleven translocation-1, tet oncogene 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDQ8NFU7
  • Protein Name
    Methylcytosine dioxygenase TET1
  • Scientific Description
    Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Hsu FM, Wu QY, Fabyanic EB, et al. TET1 facilitates specification of early human lineages including germ cells. iScience. 2023,26(7):107191. doi: 10.1016/j.isci.2023.107191
    Read this paper
  • Lemma RB, Fleischer T, Martinsen E, et al. Pioneer transcription factors are associated with the modulation of DNA methylation patterns across cancers. Epigenetics Chromatin. 2022,15(1):13. doi: 10.1186/s13072-022-00444-9
    Read this paper
  • Lan Y, Banks KM, Pan H, et al. Stage-specific regulation of DNA methylation by TET enzymes during human cardiac differentiation. Cell Rep. 2021,37(10):110095. doi: 10.1016/j.celrep.2021.110095
    Read this paper
  • Chen W, Liu N, Shen S, et al. Fetal growth restriction impairs hippocampal neurogenesis and cognition via Tet1 in offspring. Cell Rep. 2021,37(5):109912. doi: 10.1016/j.celrep.2021.109912
    Read this paper
  • Williamson SM, Ingelson-Filpula WA, Hadj-Moussa H, et al. Epigenetic underpinnings of freeze avoidance in the goldenrod gall moth, Epiblema scudderiana. J Insect Physiol. 2021,134:104298. doi: 10.1016/j.jinsphys.2021.104298
    Read this paper
  • Kim H, Kang Y, Li Y, et al. Ten-eleven translocation protein 1 modulates medulloblastoma progression. Genome Biol. 2021,22(1):125. doi: 10.1186/s13059-021-02352-9
    Read this paper
  • Wan F, Tang YW, Tang XL, et al. TET2 mediated demethylation is involved in the protective effect of triptolide on podocytes. Am J Transl Res. 2021,13(3):1233-1244.
    Read this paper
  • Skrajna A, Goldfarb D, Kedziora KM, et al. Comprehensive nucleosome interactome screen establishes fundamental principles of nucleosome binding. Nucleic Acids Res. 2020,48(17):9415-9432. doi: 10.1093/nar/gkaa544
    Read this paper
  • Peng C, Hou ST, Deng CX, et al. Function of DHX33 in promoting Warburg effect via regulation of glycolytic genes. J Cell Physiol. 2021,236(2):981-996. doi: 10.1002/jcp.29909
    Read this paper
  • Feng W, Chen S, Wang J, et al. DHX33 Recruits Gadd45a To Cause DNA Demethylation and Regulates a Subset of Gene Transcription. Mol Cell Biol. 2020,40(13). doi: 10.1128/MCB.00460-19
    Read this paper

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Figure 1. Western blot analysis of TET1 using anti-TET1 antibody (A00603-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: monkey Cos-7 whole cell lysates, Lane 6: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TET1 antigen affinity purified polyclonal antibody (Catalog # A00603-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TET1 at approximately 235KD. The expected band size for TET1 is at 235KD.
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